Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent

Mangroves are complex, unique, and dynamic ecosystems that host organisms have special physiological adaptations to fluctuations in salinity, temperature, pH. Gastropods an important role the mangrove ecosystem for food cycles webs by helping provide nutrients micro-habitats microbes. Micro-fungi isolated from productive sources of enzymes bioactive compounds such as antibacterial antifungal. In this study a explored enzymatic anti-vibrio activity fungi associated with marine gastropod Cerithidea cingulata Lottia scabra. collected three forests North Sulawesi, Indonesia. The specimens were inoculated on malt extract agar (MEA) using tapping method. Enzymatic assays conducted applying plug Positive effects fungal Vibrio indicated clear zones formed around isolates. A total 13 species C. (19MB-C5 19MBa-A4) L. scabra (19MT-07 19MT-18). Most isolates moderate growth rate. isolate highest cellulolytic, proteolytic, anti-vibriosis (Vibrio parahaemolyticus, V. harveyi, vulnificus, alginolyticus), derived 19MB-C5 was closely related Aspergillus niger, revealed molecular analysis universal primers ITS1 ITS4 (similarity value 99%). A. niger 19MB-C5-3 has potential bioremediation agent can be applied aquaculture.